DNA Ladder

10 bp DNA Ladder

Description:

10 bp DNA Ladder is designed for unique sizing of PCR products and other double stranded DNA fragments in Agarose or Non Denaturing Polyacrylamide Gels. It is step ladder with 10 bp differences between the DNA fragments. It consists of purified and blunt ended basic unit repeats of 10 bases. This not use for DNA quantification. 10 bp DNA Ladder is supplied with 6X Gel Loading Dye.

 

 

50 bp DNA Ladder-

Description:

50 bp DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on Agarose or Polyacrylamide Gels. The ladder is supplied with 6X Gel Loading Dye.

 

1 kb DNA Ladder –   Concentration: 0.5 mg/ml (50 μg)    

The 1 kb DNA Ladder is prepared from vector DNA digested to completion with appropriate restriction enzymes to yield bands ranging from 250 bp to 10 kb, suitable for use as molecular weight standards for agarose gel electrophoresis. The ladder is composed to 14 chromatography-purified individual DNA fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500 and 250.

    Storage buffer (TE buffer) –  10 mM Tris-HCl (pH 7.6), 1 mM EDTA

    Storage temperature – Store at -20oC. For frequent use divide in aliquots to avoid

multiple freeze/thaw cycles, or store at 4oC in the presence of loading buffer

(stable for 4 months).                         

    Protocol

  • Prepare loading mixture (for the 5 mm gel lane*):
  • Step 1: Mix gently
  • Step 2: Load 5 μl per 5 mm gel lane
  • Do not heat
  • Load onto the agarose gel
  • Visualise DNA by staining with ethidium bromide or with SYBR® Green I.

  *The mixture should be scaled up or down, depending on the width of the gel. Use 0.1 μg of DNA ladder/mm of lane.